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mmgA [2019-08-07 10:29:34]
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mmgA [2019-08-07 10:29:34]

degradative acetoacetyl-CoA thiolase
Locus
BSU_24170
Isoelectric point
5.90
Molecular weight
41.02 kDa
Protein length
393 aa Sequence Blast
Gene length
1182 bp Sequence Blast
Function
mother cell metabolism, leucine utilization
Product
degradative acetoacetyl-CoA thiolase
Essential
no
E.C.
2.3.1.9
Synonyms
yqiL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    2,512,861 2,514,042

    The protein

    Catalyzed reaction/ biological activity

  • 2 acetyl-CoA = CoA + acetoacetyl-CoA PubMed

    • Protein family

  • thiolase-like superfamily (according to UniProt)

    • Paralogous protein(s)

  • YhfS, FadA

    • Structure

  • 3SS6 (the protein of B. anthracis, 44% identity, 73% similarity)

    • Expression and Regulation

    Operons

    Genes
    mmgA-mmgB-mmgC-mmgD-mmgE-mmgF
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed
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    Biological materials

    Mutant

  • BKE24170 (mmgA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTTCACCTCATTCAG, downstream forward: _UP4_TAAAACATGATGAAAAAGGG
  • BKK24170 (mmgA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTTCACCTCATTCAG, downstream forward: _UP4_TAAAACATGATGAAAAAGGG

    • References

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